protein-expression-and-purification-mammalian-cells-hek-293-mt-pa

Acid phosphatase detection heavily relies on determining the concentration of tartrate-resistant acid phosphatase (TRAP) in the sample. Hence, sample preparation is very crucial and it should be done strictly as per kit manufacturer instructions to avoid any inconsistency and poor sensitivity

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using Laemmli Lysis-buffer to perform Protein isolation Bacteria - Clostridium difficile

Get tips on using Laemmli Lysis-buffer to perform Protein isolation Bacteria - Bordetella pertussis

Get tips on using 2-D Quant Kit to perform Protein quantification Colorimetric method

Get tips on using CelLytic™ B Cell Lysis Reagent to perform Protein isolation Bacteria - Fremyella diplosiphon

Get tips on using CelLytic™ B Cell Lysis Reagent to perform Protein isolation Bacteria - Synechococcus elongatus

Get tips on using CelLytic™ B Cell Lysis Reagent to perform Protein isolation Bacteria - Salmonella enterica