ELISA (kit) Human Serum Cytokine measurements (Multiplex assay) -NA-

Get tips on using PrimeScript™ 1st strand cDNA Synthesis Kit to perform cDNA synthesis Tissue

Get tips on using PrimeScript™ 1st strand cDNA Synthesis Kit to perform cDNA synthesis Bacteria

Get tips on using ProtoScript® First Strand cDNA Synthesis Kit to perform cDNA synthesis Bacteria

Get tips on using Pierce™ LAL Chromogenic Endotoxin Quantitation Kit to perform AAA for reviews

Get tips on using QuikChange Site-Directed Mutagenesis Kit, 10 Rxn to perform AAA for reviews

Get tips on using RoboGene® HDV RNA Quantification Kit 2.0 to perform RNA quantification qPCR

Get tips on using PicoGreen® dsDNA Quantitation Reagent and Kit to perform DNA quantification Blood

Get tips on using Staphylococcal Enterotoxin Reversed Passive Latex Agglutination Kit (SET- RPLA) to perform Cell Culture Contamination Detection Kit Bacteria

miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time consuming, but provide a more permanent expression of RNAi in the cells, and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines. When using oligos, the ideal concentration lies between 10-50nM for effective transfection.

Get tips on using GFP-CERTIFIED® Apoptosis/Necrosis detection kit to perform Necrosis SK-BR-3