shRNA gene silencing Mouse Prostate cancer cell lines (DU145 and PC3)

Get tips on using NFκB p65 Antibody (F-6): sc-8008 to perform Immunohistochemistry Mouse - NFκB / p65

Get tips on using β-catenin Antibody (E-5): sc-7963 to perform Immunohistochemistry Mouse - β-Catenin

Get tips on using Lab Vision™ Ki-67, Rabbit Monoclonal Antibody to perform Immunohistochemistry Mouse - Ki67

Get tips on using Estrogen Receptor alpha Antibody (F-10): sc-8002 to perform Immunohistochemistry Mouse - ERα

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using Anti-Collagen VII antibody [LH7.2] to perform Immunohistochemistry Collagen VII antibody [LH7.2] - Mouse Human -NA-

Get tips on using TransIT-TKO Transfection Reagent to perform siRNA / RNAi /miRNA transfection Mouse - Primary Splenocytes Polymer / lipid

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using pET-21b(+)-mTNFα to perform Protein Expression Prokaryotic cells - E. coli mouse TNF-(α)

Get tips on using Type-it Fast SNP Probe PCR Kit (4000) to perform Cell line authentication Peripheral blood lymphocytes