ChIP H3K27me3 Human Goat

Get tips on using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005 to perform ChIP Mouse - Kidney

Get tips on using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005 to perform ChIP Mouse - Brain

Get tips on using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Agarose Beads) #9004 to perform ChIP Mouse - Panc02

Get tips on using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Agarose Beads) #9004 to perform ChIP Mouse - BMDCs

Get tips on using Human Synoviocyte Growth Medium to perform Mammalian cell culture media HFLS

Get tips on using Pan T Cell Isolation Kit, human to perform Cell Isolation Human T cells

Get tips on using Human ICAM1 ELISA Kit (CD54) (ab100640) to perform ELISA Human - ICAM-1/CD54

miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time consuming, but provide a more permanent expression of RNAi in the cells, and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines. When using oligos, the ideal concentration lies between 10-50nM for effective transfection.

miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time consuming, but provide a more permanent expression of RNAi in the cells, and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines. When using oligos, the ideal concentration lies between 10-50nM for effective transfection.

Get tips on using PE anti-human CD96 (TACTILE) Antibody to perform Flow cytometry Anti-bodies Human - CD96