Protein expression and purification Bacteria Bacillus subtilis

Get tips on using pPICZα A, B, & C Pichia Vectors to perform Protein expression and purification Yeast - Pichia pastoris hmPRα

Get tips on using LB Broth to perform Bacterial cell culture media Bacillus anthracis

Get tips on using pPICZα A, B, & C Pichia Vectors to perform Protein expression and purification Yeast - Pichia pastoris EDIII-D1

Get tips on using Genomic DNA MiniSpin: Bacteria to perform DNA isolation / purification Bacteria - Gram positive Pseudomonas

Get tips on using mericon DNA Bacteria Kit (100) to perform DNA isolation / purification Bacteria - Gram negative Salmonella typhi

Get tips on using Difco™ Brain Heart Infusion Agar, BD to perform Bacterial cell culture media Bacillus anthracis

Generally it has been difficult to isolate high-quality RNA from yeast because of problems disrupting the cells. Use of enzymes to disrupt cell wall can alter gene expression profiles. Therefore, physical disruption can result in high quality RNA for all downstream processing. Use of DNAse and proteinase K will remove traces of DNA contamination and proteins respectively.