shRNA gene silencing Mouse Prostate cancer cell lines (DU145 and PC3)

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary mouse pulmonary artery smooth muscle cells

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using QIAGEN Proteinase K (10 ml) to perform RNA isolation / purification Cells - primary mouse morula cells

Get tips on using F4/80 antibody | Cl:A3-1 to perform Flow cytometry Anti-bodies Mouse - F4/80

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Mouse Adrenal glands

Get tips on using Hop Antibody (E-1): sc-398703 to perform Flow cytometry Anti-bodies Mouse - HOPX

Get tips on using CD4 Monoclonal Antibody (GK1.5), eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD4

Get tips on using CD74 Antibody (LN-2): sc-6262 to perform Flow cytometry Anti-bodies Mouse - CD74

Get tips on using CD34 Monoclonal Antibody (RAM34), eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD34