Protein Expression Eukaryotic cells Hi5

Get tips on using Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade to perform ChIP H3K27me3 - Sheep Rat YFP Tag

Get tips on using ChIPAb+ Trimethyl-Histone H3 (Lys36) - ChIP Validated Antibody and Primer Set to perform ChIP Anti-bodies H3K36me3

Get tips on using Di-Methyl-Histone H3 (Lys27) (D18C8) XP® Rabbit mAb #9728 to perform ChIP Anti-bodies H3K27me2

Get tips on using Mono-Methyl-Histone H3 (Lys4) (D1A9) XP® Rabbit mAb #5326 to perform ChIP Anti-bodies H3K4me1

Get tips on using PureLink™ HiPure Plasmid Filter Purification Kits - for Midi and Maxi preparation of Plasmid DNA to perform Plasmid Isolation Citrobacter koseri

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA

Worked fine for transfection . High efficiency.