siRNA / miRNA gene silencing Human Melanoma cells (501 Mel and SK Mel 28)

Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Human - MDA-MB-231

Get tips on using KAPA Stranded mRNA-Seq Kit to perform RNA sequencing Human - PBMCs

Get tips on using Vimentin Antibody to perform Immunohistochemistry Vimentin - Mouse Human -NA-

Get tips on using MUC6 Polyclonal Antibody to perform Immunohistochemistry Human - MUC-6

Get tips on using Muc Glycoprotein Antibodies to perform Immunohistochemistry Human - MUC-6

Get tips on using Muc Glycoprotein Antibodies to perform Immunohistochemistry Human - Muc-5AC

Get tips on using Muc Glycoprotein Antibodies to perform Immunohistochemistry Human - Muc-2

Get tips on using Muc Glycoprotein Antibodies to perform Immunohistochemistry Human - Muc-1

Get tips on using REG1 beta Polyclonal Antibody to perform Immunohistochemistry Human - REG1

Generally isolating RNA from Gram-negative bacteria is easy, however keeping your working environment clean and RNase free (use RNase inhibitor) is essential. Some common points to keep in mind: a) Use fresh samples for isolation or store them by freezing in RNA stabilizing buffer until use. b) Choose the bacterial input amounts carefully, to ensure buffer volumes are adequate and not to overload the columns.