Site Directed Mutagenesis (SDM) Human Point mutation HepG2

Get tips on using ATP Assay to perform Cell cytotoxicity / Proliferation assay cell type - adipose stem cells

Get tips on using Iscove’s Modified Dulbecco’s Medium to perform Stem cell culture media hMegakaryoblastic cells

Get tips on using Minimum Essential Medium Eagle to perform Stem cell culture media Rat BMSC

Get tips on using mTeSR™1 to perform Stem cell culture media hESC lines H9, H1

Get tips on using Microglia Medium to perform Stem cell Differentiation media hiPSCs differentiation into Microglial-like cells

Get tips on using Gibco™IMDM to perform Stem cell Differentiation media hESCs differentiation into SFEBq

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - primary hematopoietic stem cells

Get tips on using RNeasy Micro Kit to perform RNA isolation / purification Cells - primary hematopoietic stem cells

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.