CRISPR Rat Deletion INS-1 832/13

- Found 8053 results

Get tips on using ON-TARGETplus Rat Trio (310192) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - MTLn3 Trio

Products Horizon Discovery Ltd. ON-TARGETplus Rat Trio (310192) siRNA - SMARTpool

Get tips on using ON-TARGETplus Rat Pak1 (29431) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - MTLn3 Pak1

Products Horizon Discovery Ltd. ON-TARGETplus Rat Pak1 (29431) siRNA - SMARTpool

Get tips on using ON-TARGETplus Rat Nos3 (24600) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - NPC Nos3

Products Horizon Discovery Ltd. ON-TARGETplus Rat Nos3 (24600) siRNA - SMARTpool

Get tips on using ON-TARGETplus Rat Klf15 (85497) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - NRCM Klf15

Products Horizon Discovery Ltd. ON-TARGETplus Rat Klf15 (85497) siRNA - SMARTpool

Get tips on using ON-TARGETplus Rat Atf4 (79255) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - PC12 Atf4

Products Horizon Discovery Ltd. ON-TARGETplus Rat Atf4 (79255) siRNA - SMARTpool

Get tips on using ON-TARGETplus Rat Dcp1a (361109) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - PC12 Dcp1a

Products Horizon Discovery Ltd. ON-TARGETplus Rat Dcp1a (361109) siRNA - SMARTpool

Get tips on using ON-TARGETplus Rat Lsm1 (364624) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - PC12 Lsm1

Products Horizon Discovery Ltd. ON-TARGETplus Rat Lsm1 (364624) siRNA - SMARTpool

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

RNA siRNA / miRNA gene silencing Human BCP-1 LYVE-1

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Cellular assays Live / Dead assay mammalian cells rat MSC

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Cellular assays Live / Dead assay mammalian cells rat cardiomyocytes

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms