DNA methylation profiling Gene specific profiling HepG2

Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,

Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,

Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - immortalized HepG2

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized HepG2

Get tips on using Cell Lysis Buffer (10X) to perform Protein isolation Mammalian cells - HepG2

Get tips on using PI3 Kinase p85 Antibody to perform Autophagy assay cell type - HepG2

Get tips on using Anti-mTOR antibody [53E11] to perform Autophagy assay cell type - HepG2

Get tips on using ATG5 antibody [EPR1755(2)] to perform Autophagy assay cell type - HepG2

Get tips on using SQSTM1 Antibody (D-3) to perform Autophagy assay cell type - HepG2