DNA methylation profiling Gene specific profiling Rat whole pituitary glands

Get tips on using Rat Endothelial Cell Growth Medium to perform Mammalian cell culture media RAOEC

Get tips on using Biotin Rat Anti-CD11b to perform Flow cytometry Anti-bodies Mouse - CD11b

Get tips on using Anti-LC3 (Rat) pAb to perform Autophagy assay cell type - MLO-Y4

Get tips on using Anti-LC3 (Rat) pAb to perform Autophagy assay cell type - MLO-Y4

Get tips on using Rat PAI1 ELISA Kit (SERPINE1) (ab198509) to perform ELISA Rat - Serpin E1/PAI-1

Get tips on using Neuron specific enolase monoclonal antibody (47) to perform Immunohistochemistry Mouse - NSE

The process of RNA extraction from bacteria, in general, involves an RNA-protective, effective lysis of bacterial cell wall (which may pose difficulties). EDTA promotes loss of outer membrane to provide lysozyme with access to peptidoglycan. Another common method for cell wall lysis is mechanical disruption using a homogenizer (applied for gram-positive bacteria and some strains of gram-negative bacteria). Following lysis, it is necessary to disrupt protein-nucleic acid interactions, which can be achieved by adding sodium dodecyl sulfate (SDS). Next step involves using phenol-chloroform-isoamyl alcohol extraction, where RNA can be obtained from the bottom organic phase, the top phase consists of DNA and the interphase contains proteins. Isoamyl alcohol is an inert and optional addition to this mixture and is added as an anti-foaming reagent to reduce the interphase. Following RNA extraction, the samples should be checked for its quality by gel electrophoresis (23S and 16S rRNAs and 5s rRNA and tRNA bands) or UV spectrophotometric or fluorescence methods.

Get tips on using Rapid Sequencing Kit to perform Whole Genome Amplification Human

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.