Protein expression and purification Bacteria Origami™ B (DE3)

Get tips on using pFastBac1- B/Brisbane/60/2008-NP to perform Protein Expression Eukaryotic cells - S. frugiperda Influenza NP

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Get tips on using JetFlex™ Genomic DNA Purification Kit to perform DNA isolation / purification Bacteria - Gram positive Actinomycytes

Get tips on using HiPurA™ Streptomyces DNA Purification Kit to perform DNA isolation / purification Bacteria - Gram positive Actinomycytes

Get tips on using Easy-DNA™ gDNA Purification Kit to perform DNA isolation / purification Bacteria - Gram negative Haemophilus influenzae

Get tips on using Easy-DNA™ gDNA Purification Kit to perform DNA isolation / purification Bacteria - Gram negative Pseudomonas aeruginosa

Get tips on using HiPurA™ Streptomyces DNA Purification Kit to perform DNA isolation / purification Bacteria - Gram positive Streptomyces. Sp

I am currently using a recombinant protein which shows metal-dependent DNase activity. Is it possible to pinpoint the source of the DNase activity after protein purification? More specifically, can I ensure that the DNase activity is not because of nuclease contamination from the E.coli that might have persisted and passed with the protein of interest during purification?