RNA isolation / purification Tissue

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RNA isolation / purification Cells - primary rat dorsal root ganglion neurons Experiment

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

RNA RNA isolation / purification Cells primary rat dorsal root ganglion neurons

RNA isolation / purification Cells - primary rat retinal pigment epithelium cells Experiment

RNA RNA isolation / purification Cells primary rat retinal pigment epithelium cells

RNA isolation / purification Bacteria - Gram positive Staphylococcus aureus Experiment

The challenge in isolating RNA from S. aureus cells is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads is considered to be the better alternative.

RNA RNA isolation / purification Bacteria Gram positive Staphylococcus aureus

RNA isolation / purification Bacteria - Gram positive Streptococcus pneumoniae Experiment

The biggest problem in isolating RNA from gram-positive bacteria is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads could be the best alternative.

RNA RNA isolation / purification Bacteria Gram positive Streptococcus pneumoniae

RNA isolation / purification Bacteria - Gram positive Mycobacterium tuberculosis Experiment

RNA RNA isolation / purification Bacteria Gram positive Mycobacterium tuberculosis

RNA isolation / purification Bacteria - Gram positive Enterococcus faecalis Experiment

RNA RNA isolation / purification Bacteria Gram positive Enterococcus faecalis

RNA isolation / purification Bacteria - Gram positive Bacillus anthracis Experiment

RNA RNA isolation / purification Bacteria Gram positive Bacillus anthracis

RNA isolation / purification Bacteria - Gram positive Clostridium difficile Experiment

RNA RNA isolation / purification Bacteria Gram positive Clostridium difficile

RNA isolation / purification Bacteria - Gram positive Clostridium tetani Experiment

RNA RNA isolation / purification Bacteria Gram positive Clostridium tetani

RNA isolation / purification Bacteria - Gram positive Clostridum botulinum Experiment

RNA RNA isolation / purification Bacteria Gram positive Clostridum botulinum

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