Site Directed Mutagenesis (SDM) Human Deletion MCF-7

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized MCF 10A

Get tips on using DMEM/F-12 to perform Mammalian cell culture media MCF-10A

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - immortalized MCF 10A

Get tips on using DMEM–Dulbecco's Modified Eagle Medium to perform Mammalian cell culture media MCF-10A

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Cells - immortalized MCF 10A

Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized MCF 10A

Get tips on using MEGM-Mammary-Epithelial-Cell-Growth-Medium to perform Mammalian cell culture media MCF-10A