Site Directed Mutagenesis (SDM) Mouse Deletion

Get tips on using FITC Rat Anti-Mouse CD74 to perform Flow cytometry Anti-bodies Mouse - CD74

Get tips on using PE Rat anti-Mouse CD34 to perform Flow cytometry Anti-bodies Mouse - CD34

Get tips on using APC anti-mouse CD45 Antibody to perform Flow cytometry Anti-bodies Mouse - CD45

Get tips on using Biotin Rat Anti-Mouse CD45 to perform Flow cytometry Anti-bodies Mouse - CD45

Get tips on using PE Rat Anti-Mouse CD184 to perform Flow cytometry Anti-bodies Mouse - CD184/CXCR4

Get tips on using PE anti-mouse CD146 Antibody to perform Flow cytometry Anti-bodies Mouse - CD146/MCAM

Get tips on using PE Rat anti-Mouse CD146 to perform Flow cytometry Anti-bodies Mouse - CD146/MCAM

Get tips on using InVivoMAb anti-mouse CD16/CD32 to perform Flow cytometry Anti-bodies Mouse - CD16/CD32

Flow cytometry is an immunophenotyping technique whereby sing cell suspensions are stained for either cell surface markers or intracellular proteins by fluorescently-labelled antibodies and analyzed with a flow cytometer, where fluorescently-labelled molecules are excited by the laser to emit light at varying wavelengths, which is then detected by the instrument. There are several key criteria which are required to be kept in mind while designing a flow experiment- 1. Antibody titration (optimal dilution of antibodies should be calculated in order to avoid over- or under- saturated signals for proper detection of surface and intracellular markers), 2. Precision (3 or more replicates of the sample should be used per experiment), 3. Specificity (proper isotype controls should be included in the experiment), 4. Day-to-day variability (experiments should be repeated 3 or more times to ensure consistency and avoid variability due to flow cytometer settings), 5. Antibody interaction (Fluorescence minus one or FMO should be used, which is the comparison of signals from panel minus one antibody vs. the full panel), and 6. Antibody stability (fluorescently-labelled antibodies should be stored at 4C).