As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.
As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.
As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.
Get tips on using pET-21b-VP24 to perform Protein Expression Prokaryotic cells - E. coli VP24
Get tips on using pET-21a-IFNT to perform Protein Expression Prokaryotic cells - E. coli IFNT
Get tips on using pGS-21a Vector to perform Protein expression and purification Bacteria - Escherichia coli p19
Get tips on using pET-21b(+)-mTNFα to perform Protein Expression Prokaryotic cells - E. coli mouse TNF-(α)
Get tips on using PARP-1 monoclonal antibody (F1-23) to perform Western blotting PARP
Get tips on using pET-21a(+) DNA to perform Protein expression and purification Bacteria - Escherichia coli Prefoldin (PFD)
Get tips on using MMP-9 Antibody (2C3): sc-21733 to perform Western blotting MMP-9
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment