Site Directed Mutagenesis (SDM) Human Point mutation THP-1

Get tips on using FITC Annexin V Apoptosis Detection Kit I (RUO) to perform Apoptosis assay cell type - SMMC-7721, HEPG2

Get tips on using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® to perform RNA sequencing Mouse - ESCs (Embryonic Stem Cells)

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using SMS2 CRISPR/Cas9 KO Plasmid (m) to perform CRISPR Mouse - Deletion C2C12 Sgms2

Get tips on using SMS1 CRISPR/Cas9 KO Plasmid (m) to perform CRISPR Mouse - Deletion C2C12 Sgms1

Hello everyone! I am currently using different DNA isolation kits to extract DNA from insects. Even though I am able to successfully extract DNA I would like to maximize the yield. Do you have any tips that might help me with that even if the kits are not specifically designed for insect samples?

Get tips on using SbfI-HF® to perform Restriction Enzymes SbfI / SdaI

Get tips on using Bsp1286I NEB#R0120 to perform Restriction Enzymes Bsp1286I / SduI

Get tips on using Polybrene Infection / Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines SMMC-7721

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines SMMC-7721