Get tips on using QuantiTect Virus +ROX Vial Kit (1000) to perform PCR Quantitative real-time PCR - Viral
Get tips on using FastLane Cell SYBR Green Kit (200) to perform PCR Quantitative real-time PCR - Viral
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Get tips on using PCNA Antibody (F-2) to perform Cell cytotoxicity / Proliferation assay cell type - oral squamous cell carcinoma
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An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
Get tips on using AmpFLSTR™ Identifiler™ PCR Amplification Kit to perform Cell line authentication ML14 lymphoblastoid cell line
I have been doing multiplex PCR and qPCR separately. Is there a way to combine them in a single reaction using Sybr Green?
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