Get tips on using pMAPLe4 to perform Protein Expression Prokaryotic cells - E. coli mycobacterial Esx complex
Get tips on using pCcrtB to perform Protein Expression Prokaryotic cells - E. coli Kocuria gwangalliensis CrtB
Get tips on using pKUC4 to perform Protein Expression Prokaryotic cells - B. licheniformis PphyL′-′xynA fusion
Get tips on using pKUC3 to perform Protein Expression Prokaryotic cells - B. licheniformis PphyL′-′amyE fusion
Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.
Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.
Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.
Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.
Get tips on using pBru-prochymosin to perform Protein Expression Prokaryotic cells - E. coli calf prochymosin
Get tips on using pET24b-prochymosin to perform Protein Expression Prokaryotic cells - E. coli calf prochymosin
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment