cell-cycle-assay-human-hela

Get tips on using GeneChip® HT 3' IVT PLUS Reagent Kit to perform Microarray Human - Endometrial stromal cells Target preparation kit (Amplification + Hybridization + control)

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

Get tips on using Neogen-Brain-Heart Infusion Broth to perform Bacterial cell culture media Salmonella enterica

Get tips on using BHI-BRAIN HEART INFUSION BROTH to perform Bacterial cell culture media Listeria monocytogens

Get tips on using Silencer select_ObR siRNA to perform siRNA / miRNA gene silencing Human - MCF-7 ObR(leptin receptor/LEPR)

Get tips on using EpiTect Bisulfite Kit to perform DNA methylation profiling Whole genome profiling - OVCAR-3 human ovarian cancer

Get tips on using Attractene Transfection Reagent to perform shRNA gene silencing Human - HEK 293T CAPN5- (Calpains) cationic lipid based

Get tips on using Anti-Collagen Type VII Antibody, clone 32,-VII to perform Immunohistochemistry Collagen VII [II-32] - Mouse Human -NA-

Get tips on using stealth siRNA GIRK1/KCNJ3 to perform siRNA / miRNA gene silencing Human - MDA-MB-453 GIRK1/KCNJ3

Get tips on using stealth siRNA GIRK1/KCNJ3 to perform siRNA / miRNA gene silencing Human - MDA-MB-231 GIRK1/KCNJ3