Protein Expression Eukaryotic cells W. anomalus

Get tips on using QIAseq FX Single Cell DNA Library Kit (96) to perform Whole Genome Amplification Parasites

Get tips on using QIAseq FX Single Cell RNA Library Kit (96) to perform Whole Transcriptome Amplification Virus

Get tips on using Monoclonal Anti-Proliferating Cell Nuclear Antigen antibody produced in mouse to perform Western blotting PCNA

Get tips on using Anti-Atg5 to perform Autophagy assay cell type - Mouse white adipose tissue

Get tips on using mTeSR™1 to perform Stem cell culture media Human WA09 hESC

Get tips on using B2M siRNA to perform siRNA / miRNA gene silencing Human - hES cell line H1 (WA01) B2M

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using REPLI-g UltraFast Mini Kit (100) to perform Whole Genome Amplification Cell lines

Get tips on using Gibco™DMEM/F-12 to perform Stem cell culture media Human WA09 hESC

Get tips on using EpiTect Bisulfite Kit to perform DNA methylation profiling Whole genome profiling - mouse T-cell (CD4 / CD8)