Immunofluorscence  53BP1 [H-300]

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using FastDigest HhaI to perform Restriction Enzymes Hin6I / HinP1I / Hhal

Get tips on using HindIII-HF® to perform Restriction Enzymes HindIII

Get tips on using HhaI restriction enzyme to perform Restriction Enzymes Hin6I / HinP1I / Hhal

Get tips on using HinP1I NEB#R0124 to perform Restriction Enzymes Hin6I / HinP1I / Hhal

Get tips on using Histone H3K9ac antibody to perform ChIP H3K9Ac - Sheep Rat -NA-

Get tips on using Histone H4K20me1 antibody (mAb) to perform ChIP Anti-bodies H4K20me1

Get tips on using Histone H3K36me3 antibody (mAb) to perform ChIP Anti-bodies H3K36me3

Get tips on using Histone H3K9me3 antibody (mAb) to perform ChIP Anti-bodies H3K9me3

Get tips on using Histone H3K4me3 antibody (mAb) to perform ChIP Anti-bodies H3K27me1