PCR

Get tips on using Purified anti-Tubulin β-3 (TUBB3) Antibody (Previously Covance catalog# PRB-435P) to perform Immunohistochemistry Mouse - TUBB3

Get tips on using PureLink™ HiPure Plasmid Filter Purification Kits - for Midi and Maxi preparation of Plasmid DNA to perform Plasmid Isolation Citrobacter koseri

Get tips on using pFR2 to perform Protein Expression Prokaryotic cells - E. coli ferulic acid

Get tips on using pCW-Cas9 to perform CRISPR Human - Repression ENII-CP/X

Get tips on using pcDNA-dCas9-VP64 to perform CRISPR Human - Activation REPRIMO

Get tips on using ON-TARGETplus Human PCSK7 (9159) siRNA - Individual to perform siRNA / miRNA gene silencing Human - A253 PC-7

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

Get tips on using Monoclonal Mouse Anti-Human Progesterone Receptor (Concentrate) Clone PgR 1294 to perform Immunohistochemistry Human - PR

Get tips on using pTrcHis-TOPO to perform Protein Expression Prokaryotic cells - E. coli Murine BCO2

Get tips on using pcDNA-dCas9-p300 Core to perform CRISPR Human - Activation MASPIN