Site Directed Mutagenesis (SDM) Mouse C2C12

Get tips on using PE Rat Anti-Mouse CD184 to perform Flow cytometry Anti-bodies Mouse - CD184/CXCR4

Get tips on using PE anti-mouse CD146 Antibody to perform Flow cytometry Anti-bodies Mouse - CD146/MCAM

Get tips on using PE Rat anti-Mouse CD146 to perform Flow cytometry Anti-bodies Mouse - CD146/MCAM

Get tips on using InVivoMAb anti-mouse CD16/CD32 to perform Flow cytometry Anti-bodies Mouse - CD16/CD32

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using IntestiCult™ Organoid Growth Medium (Mouse) to perform 3D Cell Culture Media Mouse small intestinal organoids

Get tips on using Purified Rat Anti-Mouse CD117 to perform Flow cytometry Anti-bodies Mouse - CD117/c-kit

Get tips on using PE Hamster Anti-Mouse CD279 to perform Flow cytometry Anti-bodies Mouse - CD279/PD-1

Get tips on using PE Rat Anti-Mouse CD138 to perform Flow cytometry Anti-bodies Mouse - CD138/Syndecan-1