siRNA / miRNA gene silencing Human HL-60

Get tips on using siGENOME Human TBX2 (6909) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - U251 TBX2

Get tips on using siGENOME Human BRCA1 (672) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - ES2 BRCA1

Get tips on using siGENOME Human DAB2 (1601) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - A549 DAB2

Get tips on using Accell Human MYB (4602) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - LAMA84 MYB

Get tips on using Accell Human VEGFC (7424) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - LAMA84 VEGFC

Get tips on using Accell Human MYB (4602) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - K562 MYB

Get tips on using Accell Human VEGFC (7424) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - K562 VEGFC

Get tips on using Accell Human CD44 (960) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HaCaT CD44

I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method?

Get tips on using APC BrdU Flow Kit to perform Cell cycle assay human - HL-60