siRNA / miRNA gene silencing Rat RBL-2H3

Get tips on using ON-TARGETplus Rat Rhoc (295342) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - MTLn3 RhoC

Get tips on using ON-TARGETplus Rat Rhog (308875) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - MTLn3 RhoG

Get tips on using ON-TARGETplus Rat Trio (310192) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - MTLn3 Trio

Get tips on using ON-TARGETplus Rat Pak1 (29431) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - MTLn3 Pak1

Get tips on using ON-TARGETplus Rat Nos3 (24600) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - NPC Nos3

Get tips on using ON-TARGETplus Rat Klf15 (85497) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - NRCM Klf15

Get tips on using ON-TARGETplus Rat Atf4 (79255) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - PC12 Atf4

Get tips on using ON-TARGETplus Rat Dcp1a (361109) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - PC12 Dcp1a

Get tips on using ON-TARGETplus Rat Lsm1 (364624) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - PC12 Lsm1

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.