Protein Expression Eukaryotic cells W. anomalus

Get tips on using OCT4-PG1 siRNA to perform siRNA / miRNA gene silencing Human - hES cell line H1 (WA01) OCT4-PG1

Get tips on using Anti-Beclin 1 (Human) pAb to perform Autophagy assay cell type - Mouse white adipose tissue

Get tips on using Gibco™KnockOut™ DMEM to perform Stem cell culture media hESC standard maintenance with MEFs

Get tips on using Gibco™DMEM/F-12 to perform Stem cell culture media hESC standard maintenance with MEFs

Get tips on using Dulbecco’s Modified Eagle’s Medium - high glucose to perform Stem cell culture media Human WA09 hESC

Get tips on using EZ DNA Methylation kit to perform DNA methylation profiling Whole genome profiling - mouse T-cell (CD4 / CD8)

Get tips on using MethylFlash Hydroxymethylated DNA Quantification Kit to perform DNA methylation profiling Whole genome profiling - human germ cell cancer

Get tips on using MethylFlash Methylated DNA 5-mC Quantification Kit to perform DNA methylation profiling Whole genome profiling - human germ cell cancer

Get tips on using ON-TARGETplus Human LIN28A (79727) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - hES cell line H1 (WA01) LIN28

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.