siRNA / miRNA gene silencing Human Primary Endometrial Stromal Cells IGFBP1 (Insuline-like growth factor binding protein-1)

Get tips on using LC3B Antibody to perform Autophagy assay cell type - THP 1

Get tips on using Tissue Extraction Reagent I to perform Protein isolation Tissue - Mouse liver tissue

Get tips on using GeneArt™ Site-Directed Mutagenesis PLUS System to perform Site Directed Mutagenesis (SDM) Human - Insertion SKOV3 miR-134

Get tips on using 1kb DNA Step Ladder to perform DNA Ladder 1 kb

Get tips on using BenchTop 1kb DNA Ladder to perform DNA Ladder 1 kb

Get tips on using GeneArt™ Site-Directed Mutagenesis System to perform Site Directed Mutagenesis (SDM) Mouse - 3T3-L1 S6 kinase 1

Get tips on using CelLytic™ M to perform Protein isolation Bacteria - Staphylococcus aureus

The challenge in isolating RNA from S. aureus cells is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads is considered to be the better alternative.

Get tips on using GeneJET Plasmid Miniprep Kit to perform Plasmid Isolation Proteus mirabilis

Get tips on using TAGZyme pQE Vector Set to perform Protein tag Expression of His-tagged proteins