siRNA / miRNA gene silencing Human SUIT-2

Get tips on using pHYαCGTd4 to perform Protein Expression Prokaryotic cells - B. subtilis Pullulanase

Get tips on using pGPA to perform Protein Expression Prokaryotic cells - B. subtilis Abaecin

Get tips on using Trichloroacetic acid to perform Protein isolation Bacteria - Bacillus subtilis

Get tips on using ApopTag® Fluorescein In Situ Apoptosis Detection Kit to perform TUNEL assay cell type - Islets of langerhans (Beta cells)

Get tips on using pGETS118 to perform Protein Expression Prokaryotic cells - B. subtilis cellulosomal complexes

Get tips on using pHYαCGT7 to perform Protein Expression Prokaryotic cells - B. subtilis α-CGTase

Get tips on using pHYαCGT1 to perform Protein Expression Prokaryotic cells - B. subtilis β-CGTase

Get tips on using pAF3 to perform Protein Expression Prokaryotic cells - B. subtilis PHY US417

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.