Protein Expression Eukaryotic cells N. benthamiana

Get tips on using NruI-HF® to perform Restriction Enzymes NruI / Bsp68I

Get tips on using NruI restriction enzyme to perform Restriction Enzymes NruI / Bsp68I

Get tips on using Anti-Notch1 antibody (ab27526) to perform Immunohistochemistry Human - Notch1

Get tips on using NdeI (10 U/µL) to perform Restriction Enzymes NdeI

Get tips on using NheI (10 U/µL) to perform Restriction Enzymes NheI

Get tips on using NotI (10 U/µL) to perform Restriction Enzymes NotI

Get tips on using NcoI (10 U/µL) to perform Restriction Enzymes NcoI

Get tips on using Anti-NLRP3 antibody (ab214185) to perform Western blotting NLRP3

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.