siRNA / miRNA gene silencing Human 501 Mel and SK Mel 28 FANCD2

I would like to excise a large strand of DNA and insert a new one using CRISPR. My problem is that my strand will be a little over 1kb and I am not sure if this is going to be a limiting factor. Also, how long should the homology arms be for a region of this size?

Get tips on using Alexa Fluor 700-labeled anti-CD16 to perform Flowcytometry CD16 - Mouse /IgG1, kappa Human Alexa Fluor 700

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Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human airway epithelial cells

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