siRNA / miRNA gene silencing Human Primary Human Aortic Endothelial Cells GLO-1

Get tips on using GenElute™ Mammalian Total RNA Miniprep Kit to perform RNA isolation / purification Cells - immortalized A2780

Get tips on using GenElute™ Mammalian Total RNA Miniprep Kit to perform RNA isolation / purification Cells - immortalized HeLa

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized GL261

Get tips on using PolyFect Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines Chang Liver cells

Get tips on using KGMTM Gold Keratinocyte Growth Medium BulletKitTM to perform Mammalian cell culture media HEK

Get tips on using Zombie UV™ Fixable Viability Kit to perform Live / Dead assay mammalian cells - Spleen cells

Get tips on using Gibco™ DMEM, high glucose to perform Stem cell culture media hMyoblasts cells

A key signature for necrotic cells is the permeabilization of the plasma membrane. Necrosis can be quantified by several cellular and biochemical assays. When studied minutely, it reveals the difficulty in confirmation in secondary induction of necrosis in apoptotic cells. Apoptotic cells are being analyzed to shift to necrotic status owing to membrane permeability at later stages, and thus, discrimination of two cell death becomes critical. Therefore, it is crucial to use a necrosis detection kit or a defined procedure to analyze this unprogrammed form of death in response to immense chemical and physical insults.

Get tips on using pSB 130 to perform Protein Expression Prokaryotic cells - A. tumefaciens ELP-intein

Get tips on using Gibco™ DMEM, high glucose to perform Stem cell culture media Mouse myoblasts cells