pcr-hot-start-pcr

Get tips on using CpGenome Universal DNA Modification Kit to perform DNA methylation profiling Gene specific profiling - Ca Ski L2

Get tips on using EZ DNA Methylation-Gold Kit to perform DNA methylation profiling Gene specific profiling - Ca Ski TIMP2

Get tips on using Imprint® DNA Modification Kit to perform DNA methylation profiling Gene specific profiling - Caco-1 Bax

Get tips on using EZ DNA Methylation-Gold Kit to perform DNA methylation profiling Gene specific profiling - MCF-7 BMP2

Get tips on using SensiFAST™ Probe No-ROX One-Step Kit to perform RNA quantification qPCR

Get tips on using Anti-p62/SQSTM1 antibody produced in rabbit to perform Autophagy assay cell type - HeLa

Get tips on using Anti-LC3 antibody produced in rabbit to perform Autophagy assay cell type - SK-MEL-28

Get tips on using EZ DNA Methylation kit to perform DNA methylation profiling Whole genome profiling - OVCAR-3 human ovarian cancer

Get tips on using EZ DNA Methylation kit to perform DNA methylation profiling Whole genome profiling - mouse T-cell (CD4 / CD8)

What is the optimal concentration for primers in qPCR? My total volume is 20μl per reaction.