siRNA / miRNA gene silencing Human CRL-5915

Get tips on using MEBMTM Mammary Epithelial Cell Growth Basal Medium to perform 3D Cell Culture Media Mouse primary breast ephitelial cells-Mammospheres

Get tips on using Vascular Cell Basal Medium to perform Mammalian cell culture media HCAEC

Get tips on using Smooth Muscle Cell Medium to perform Mammalian cell culture media HAOSMC

Get tips on using Canine Endothelial Cell Media to perform Mammalian cell culture media CnAOEC

Get tips on using Bovine Endothelial Cell Media to perform Mammalian cell culture media BAOEC

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using MEBMTM Mammary Epithelial Cell Growth Basal Medium to perform 3D Cell Culture Media Mouse primary breast cancer ephitelial cells-Mammospheres

Get tips on using EGMTM-2 Endothelial Cell Growth Medium-2 BulletKit to perform Stem cell culture media hLung Mesenchymal Stem Cells (MSCs)

Get tips on using EBMTM-2 Endothelial Cell Growth Basal Medium-2 to perform Stem cell culture media Cord blood-derived endothelial cells(hCBiPS2)

Get tips on using Rat Endothelial Cell Growth Medium to perform Mammalian cell culture media RAOEC