rna-isolation-purification-cells-immortalized-cal-27

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.

Get tips on using mirVana™ miRNA Isolation Kit, with phenol to perform RNA isolation / purification Cells - immortalized Calu-3

DNA isolation and purification is the first critical step in sample preparation that helps ensure optimal performance of downstream assays like PCR, microarrays, and sequencing. Failure in yielding high-quality DNA would be the major reason that DNA doesn't work for the downstream application. To circumvent this, one should follow the recommended storage conditions to minimize DNA degradation by nucleases and shouldn't overload the purification system.

Generally isolating RNA from Gram-negative bacteria is easy, however keeping your working environment clean and RNase free (use RNase inhibitor) is essential. Some common points to keep in mind: a) Use fresh samples for isolation or store them by freezing in RNA stabilizing buffer until use. b) Choose the bacterial input amounts carefully, to ensure buffer volumes are adequate and not to overload the columns.

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Cells - immortalized SK-BR-3

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Cells - immortalized ZR-75-1

Get tips on using ReliaPrep™ RNA Miniprep Systems to perform RNA isolation / purification Cells - immortalized MDA-MB-468

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Cells - immortalized MDA-MB-231

Get tips on using ReliaPrep™ RNA Miniprep Systems to perform RNA isolation / purification Cells - immortalized MDA-MB-231

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Cells - immortalized MDA-MB-157