siRNA / miRNA gene silencing Human hES cell line H1 (WA01)

Get tips on using ON-TARGETplus Human PLK1 (5347) siRNA - Individual to perform siRNA / miRNA gene silencing Human - H1299 PLK-1

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

Get tips on using ON-TARGETplus Human BECN1 siRNA to perform siRNA / miRNA gene silencing Human - U251 Beclin 1

Get tips on using Silencer® GAPDH siRNA (human) to perform siRNA / miRNA gene silencing Human - Jurkat GAPDH Lipid

Get tips on using ON-TARGETplus Human ARL2 (402) siRNA - Individual to perform siRNA / miRNA gene silencing Human - HeLa ARL2

Get tips on using ON-TARGETplus Human ELMOD2 (255520) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HeLa ELMOD2

Get tips on using ON-TARGETplus Human ARL3 (403) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HeLa ARL3

Get tips on using ON-TARGETplus Human CDK1 (983) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HEK293 CDK1

Get tips on using ON-TARGETplus Human CDK2 (1017) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HEK293 CDK2

Get tips on using ON-TARGETplus Human SMU1 (55234) siRNA - Individual to perform siRNA / miRNA gene silencing Human - HeLa SMU1