Protein Expression Prokaryotic cells B. bifidum

Get tips on using LB Broth, Lennox (Granulated) to perform Bacterial cell culture media Pseudomonas aeruginosa

Get tips on using IBI’s Todd-Hewitt Broth to perform Bacterial cell culture media Escherichia coli

Get tips on using BD Tryptic Soy Agar to perform Bacterial cell culture media Staphylococcus aureus

Get tips on using IBI’s Todd-Hewitt Broth to perform Bacterial cell culture media Salmonella Typhi

Get tips on using BRAIN HEART INFUSION AGAR to perform Bacterial cell culture media Streptococcus pneumoniae

Get tips on using Thioglycolate Broth with Resazurine to perform Bacterial cell culture media Clostridium tetani

Get tips on using B2M siRNA to perform siRNA / miRNA gene silencing Human - hES cell line H1 (WA01) B2M

Get tips on using Agilent DNA 1000 Kit Bioanalyzer DNA Analysis Part Number:5067-1504 to perform Cell line authentication Human lung carcinoma cell line NCI-H1299

Get tips on using APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU to perform Apoptosis assay cell type - PANC-1

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.