shRNA gene silencing Mouse Prostate cancer cell lines (DU145 and PC3) CD24

Get tips on using Ovation® RNA-Seq System V2 to perform RNA sequencing Mouse - C2C12

Get tips on using TruSeq RNA Library Prep Kit v2 to perform RNA sequencing Mouse - Microglia

Get tips on using TruSeq RNA Library Prep Kit v2 to perform RNA sequencing Mouse - RAW264.7

Get tips on using OSTEOPONTIN (O-17) ANTI-HUMAN RABBIT IGG AFFINITY PURIFY to perform Immunohistochemistry Mouse - Spp1/OPN

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using Notch1 (D1E11) XP® Rabbit mAb #3608 to perform Immunohistochemistry Mouse - Notch1

Get tips on using Cleaved Notch1 (Val1744) (D3B8) Rabbit mAb #4147 to perform Immunohistochemistry Mouse - Notch1

Get tips on using ChIP-IT® Express Chromatin Immunoprecipitation Kits to perform ChIP Mouse - Brain

Get tips on using ChIP-IT® Express Chromatin Immunoprecipitation Kits to perform ChIP Mouse - HT22

Get tips on using ChIP-IT® Express Chromatin Immunoprecipitation Kits to perform ChIP Mouse - NIH3T3