rna-isolation-purification-cells-primary-canine-peripheral-blood-mononuclear-cells

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Get tips on using ISOLATE II PCR and Gel kit to perform DNA gel extraction / PCR product purification Product size < 15Kb

Products Bioline ISOLATE II PCR and Gel kit

Get tips on using HiTrap Q FF anion exchange chromatography column to perform Protein expression and purification Bacteria - Bacillus subtilis GCSF

Products GE Healthcare Life Sciences HiTrap Q FF anion exchange chromatography column

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay luciferase SW480 human colon cancer cell line

Get tips on using RosetteSep™ HLA B Cell Enrichment Cocktail to perform Cell Isolation HLA B Cell

Products STEMCELL technologies RosetteSep™ HLA B Cell Enrichment Cocktail

Get tips on using EasySep™ HLA B Cell Enrichment Kit to perform Cell Isolation HLA B Cell

Products STEMCELL technologies EasySep™ HLA B Cell Enrichment Kit

Get tips on using EasySep™ HLA T Cell Enrichment Kit to perform Cell Isolation HLA T Cell

Products STEMCELL technologies EasySep™ HLA T Cell Enrichment Kit

Get tips on using RosetteSep™ HLA T Cell Enrichment Cocktail to perform Cell Isolation HLA T Cell

Products STEMCELL technologies RosetteSep™ HLA T Cell Enrichment Cocktail

Get tips on using RNAprotect Cell Reagent (250 ml) to perform RNA stabilization Cell lines

Products Qiagen RNAprotect Cell Reagent (250 ml)

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

Cellular assays Cell cycle assay mouse RAW 264.7

Get tips on using EasySep™ Release Human CD19 Positive Selection Kit to perform Cell Isolation B cell

Products STEMCELL technologies EasySep™ Release Human CD19 Positive Selection Kit

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