rna-isolation-purification-tissue-rat-spinal-cord

Get tips on using Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate to perform Flowcytometry Secondary Antibody - Goat Rabbit Alexa Fluor 488

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Cardiomyocytes

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Human prenatal cardiac progenator cells

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using Rabbit anti-calretinin to perform Immunohistochemistry Mouse - Calb2

Get tips on using Rapid Sequencing Kit to perform Whole Genome Amplification Human

Get tips on using FOXP3 Rabbit Polyclonal Antibody to perform Western blotting FOXP3

Get tips on using Anti-CCSP - Rabbit to perform Flow cytometry Anti-bodies Mouse - CCSP

Get tips on using LC3B Rabbit Antibody to perform Autophagy assay cell type - HEK 293

Get tips on using LC3B Rabbit Antibody to perform Autophagy assay cell type - SH-SY5Y