cell-cycle-assay-human-hela

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Get tips on using QuikChange II XL Site-Directed Mutagenesis Kit, 10 Rxn to perform Site Directed Mutagenesis (SDM) Human - Deletion PC-3 AGR2

Products Agilent Technologies QuikChange II XL Site-Directed Mutagenesis Kit, 10 Rxn

Get tips on using QuikChange II XL Site-Directed Mutagenesis Kit, 10 Rxn to perform Site Directed Mutagenesis (SDM) Human - Deletion MCF-7 AHR

Products Agilent Technologies QuikChange II XL Site-Directed Mutagenesis Kit, 10 Rxn

Get tips on using QuikChange II XL Site-Directed Mutagenesis Kit, 10 Rxn to perform Site Directed Mutagenesis (SDM) Human - Point mutation H1299 PDHA1

Products Agilent Technologies QuikChange II XL Site-Directed Mutagenesis Kit, 10 Rxn

Get tips on using Ambion™ RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE to perform RNA isolation / purification Tissue - human kidney tissue

Products Fisher Scientific Ambion™ RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE

Get tips on using Anti-Cytokeratin AE1/AE3 Antibody, recognizes acidic & basic cytokeratins, clone AE1/AE3 to perform Flow cytometry Anti-bodies Human - Keratin

Products Sigma-Aldrich Anti-Cytokeratin AE1/AE3 Antibody, recognizes acidic & basic cytokeratins, clone AE1/AE3

Get tips on using Pre-designed and validated siRNA against gene IGFBP1 to perform siRNA / miRNA gene silencing Human - Primary Endometrial Stromal Cells IGFBP1 (Insuline-like growth factor binding protein-1)

Products Thermo Fisher Scientific Pre-designed and validated siRNA against gene IGFBP1

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

RNA RNA isolation / purification Cells primary hematopoietic stem cells

Get tips on using Brain heart infusion bhi agar 20 plates BD Difco BBL to perform Bacterial cell culture media Bordetella bronchiseptica

Products Science Equip Brain heart infusion bhi agar 20 plates BD Difco BBL

Get tips on using DuoSet® ELISA Development Systems to perform ELISA (kit) IL-6, IL-1β, IL-8 and TNF-α - -NA- Human -NA-

Products R&D system, Minneapolis, MN, USA DuoSet® ELISA Development Systems

Get tips on using QuikChange II XL Site-Directed Mutagenesis Kit, 10 Rxn to perform Site Directed Mutagenesis (SDM) Human - Deletion K562 c-Myb gene

Products Agilent Technologies QuikChange II XL Site-Directed Mutagenesis Kit, 10 Rxn

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