siRNA / miRNA gene silencing Human CRL-5915

Get tips on using Gibco™ MEM α, Nucleosides to perform Stem cell culture media Periodontal ligament stem cells (PDLSCs)

Get tips on using Gibco™ DMEM, high glucose to perform Stem cell culture media Periodontal ligament stem cells (PDLSCs)

Get tips on using Gibco™Neurobasal™ Medium to perform Stem cell culture media GBM patient-derived stem cells

Get tips on using DeadEnd™ Colorimetric TUNEL System to perform TUNEL assay cell type - 3T3 L1 mouse adipose tissue

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using DMEM with Glucose and L-Glutamine to perform 3D Cell Culture Media U87MG cells- glioblastoma spheres

Get tips on using DMEM/F-12, no phenol red to perform 3D Cell Culture Media BT-549 cells-Mammospheres

Get tips on using DMEM/F-12 PLUS Basal Medium to perform 3D Cell Culture Media BT-549 cells-Mammospheres

Get tips on using Gibco DMEM/F-12, HEPES to perform 3D Cell Culture Media Mouse primary mammary ephitelial cells- organoids

Get tips on using DMEM (Dulbecco's Modified Eagle's medium) to perform Stem cell culture media Cord blood-derived endothelial cells(hCBiPS2)