PCR Quantitative real-time PCR

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel

Get tips on using EMBacY#5 to perform Protein Expression Eukaryotic cells - Hi5 Soluble G protein (Hendra Virus)

Get tips on using pTSara-NatB to perform Protein Expression Prokaryotic cells - E. coli N-terminal acetyltransferase B

Get tips on using pET21a-alpha-synuclein to perform Protein Expression Prokaryotic cells - E. coli alpha-synuclein

Get tips on using pASK-TS-MCoTI to perform Protein Expression Prokaryotic cells - E. coli SFTI-1

Get tips on using pQE-30 to perform Protein Expression Prokaryotic cells - E. coli Guinea Pig TNF-Alpha

Get tips on using pEV-UDP-E to perform Protein Expression Prokaryotic cells - E. coli 2-epimerase

Get tips on using pET31b(+)- LPcin-YK3 to perform Protein Expression Prokaryotic cells - E. coli LPcin-YK3

Get tips on using pLD‐CoHSA‐Trxm to perform Protein Expression Prokaryotic cells - E. coli Tobacco Trxm

Get tips on using pLD‐CoHSA‐Trxf to perform Protein Expression Prokaryotic cells - E. coli Tobacco Trxf