RNA isolation / purification Cells Cancer cell lines

Get tips on using QIAGEN Plasmid Kits to perform Plasmid Isolation Shigella flexneri

Get tips on using HiSpeed Plasmid Kits to perform Plasmid Isolation Bacillus group

Get tips on using QuickLyse Miniprep Kit (250) to perform Plasmid Isolation Enterobacteriaceae

Short hairpin or small hairpin RNA (shRNA) is artificial RNA, which has a hairpin loop structure, and uses inherent microRNA (miRNA) machinery to silence target gene expression. This is called RNA interference (RNAi). These can be delivered via plasmids or viral/bacterial vectors. Challenges in shRNA-mediated gene silencing include: 1. Off-target silencing, 2. Packaging shRNA encoding lentivirus, and 3. Stable transduction in cells. RNAi have been designed to have anywhere from 19-27 bs, but the most effective design has 19 bp. In case commercial shRNAs are not available, potential target sites can be chosen within exon, 5’- or 3’ UTR, depending on which splice variants of the gene are desired. One should use the latest algorithms and choose at least two different sequences, targeting different regions, in order to have confidence in overcoming off-target effects. A BLAST search after selecting potential design will eliminate potential off-target sequences. For the second challenge, sequencing the vector using primers for either strand (50-100 bp upstream) is suggested, along with using enzymatic digestion on agarose gel for the vector. Next, once the shRNA-containing vector is packaged in a virus, it is important to check the viral titer before transduction. Finally, using a marker in the lentiviral vector (fluorescent protein or antibiotic resistance), along with qPCR for target gene expression can help in determining efficacy of transduction and shRNA on its target site.

Get tips on using PowerPlex® 16 System to perform Cell line authentication Bladder carcinoma cell line T24

Get tips on using PowerPlex® 16 System to perform Cell line authentication Liver carcinoma cell line HepG2

Get tips on using PowerPlex® Y23 System to perform Cell line authentication MDA‐MB‐435S cell line

Get tips on using SNP Trace™ Panel to perform Cell line authentication MDA‐MB‐435S cell line

Get tips on using SNP Trace™ Panel to perform Cell line authentication MDA‐MB‐435 cell line

Get tips on using PowerPlex® Y23 System to perform Cell line authentication MDA‐MB‐435 cell line