PCR ORNi-PCR

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Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,

DNA DNA isolation / purification Cells Primary cells Pseudomyxoma peritonei (PMP) cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary porcine primary airway epithelial cell

Products Thermo Fisher Scientific TRIzol Reagent

Get tips on using rMaj-pCHH-B to perform Protein Expression Prokaryotic cells - E. coli M. japonicus CHH-like peptide

Products Naoaki Tsutsui, Faculty of Science, Ushimado Marine Institute, O rMaj-pCHH-B

Get tips on using SV 96 Total RNA Isolation System to perform RNA isolation / purification Cells - primary porcine primary airway epithelial cell

Products Promega SV 96 Total RNA Isolation System

Get tips on using mirVana™ miRNA Isolation Kit, with phenol to perform RNA isolation / purification Cells - primary porcine primary airway epithelial cell

Products Thermo Fisher Scientific mirVana™ miRNA Isolation Kit, with phenol
pcDNA-nGP1 Product

Get tips on using pcDNA-nGP1 to perform Protein Expression Eukaryotic cells - HEK293 GP1

Products Peter D. Sun, Structural Immunology Section, Laboratory of Immun pcDNA-nGP1
pcDNA-oGP1 Product

Get tips on using pcDNA-oGP1 to perform Protein Expression Eukaryotic cells - HEK293 GP1

Products Peter D. Sun, Structural Immunology Section, Laboratory of Immun pcDNA-oGP1
pCH1-HRP Product

Get tips on using pCH1-HRP to perform Protein Expression Eukaryotic cells - P. pastoris HRP

Products Geoff P. Lin-Cereghino, Department of Biological Sciences, Unive pCH1-HRP
pCMV-spAAT Product

Get tips on using pCMV-spAAT to perform Protein Expression Eukaryotic cells - N. benthamiana CMViva

Products Karen A. McDonald, Department of Chemical Engineering and Materi pCMV-spAAT

Get tips on using pCMV-HA2/DKK1 to perform Protein Expression Eukaryotic cells - HEK293 DKK1

Products G.Y. Bao, Department of Clinical Laboratory, Yangzhou First Peop pCMV-HA2/DKK1

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