Western blot p62/SQSTM1 Mouse IgG2b Human

Get tips on using Recombinant Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537) to perform Western blotting Ubiquitin

Get tips on using Anti-HIF-1 alpha antibody - ChIP Grade (ab2185) to perform Western blotting HIF-1 alpha

Get tips on using Phospho-IKKα (Ser176)/IKKβ (Ser177) (C84E11) Rabbit mAb #2078 to perform Western blotting IKK Alpha

Get tips on using Recombinant Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870) to perform Western blotting IKK Alpha

Get tips on using Recombinant Anti-Cyclin D1 antibody [EPR2241] - C-terminal (ab134175) to perform Western blotting Cyclin D1

Get tips on using Phospho-NF-κB p105 (Ser933) (18E6) Rabbit mAb #4806 to perform Western blotting NF-kB

Get tips on using Anti-TATA binding protein TBP antibody [58C9] - Loading Control (ab61411) to perform Western blotting TBP

Get tips on using Recombinant Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] (ab138005) to perform Western blotting Jak1

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.