Get tips on using Minimum Essential Medium Eagle to perform Mammalian cell culture media Vero
Get tips on using SuperScript IV VILO Master Mix to perform cDNA synthesis Cell lines
Get tips on using SuperScript® II Reverse Transcriptase to perform cDNA synthesis Cell lines
Get tips on using RevertAid RT Reverse Transcription Kit to perform cDNA synthesis Cell lines
The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.
Get tips on using Microglia Medium to perform Stem cell Differentiation media hiPSCs differentiation into Microglial-like cells
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary porcine primary airway epithelial cell
Get tips on using DMEM/F-12 to perform 3D Cell Culture Media PLC/PRF/5 cells-spheres
Get tips on using RPMI-1640 Medium to perform Stem cell culture media hLung Mesenchymal Stem Cells (MSCs)
Get tips on using Adipogenic Induction Medium to perform Stem cell Differentiation media mPericytes differentiation into adipogenic cells
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