DNA transfection Mammalian cells Immortalized cell lines

Microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

Get tips on using B2M siRNA to perform siRNA / miRNA gene silencing Human - hES cell line H1 (WA01) B2M

Get tips on using pGL3-Basic Vector to perform Reporter gene assay luciferase - SW480 human colon cancer cell line

Get tips on using EZ-Magna ChIP™ G - Chromatin Immunoprecipitation Kit to perform ChIP Human - Glioblastoma cell line

Get tips on using pVRB2B3 to perform Protein Expression Eukaryotic cells - HEK293 hβ-defensin 2/3

Get tips on using pMAPLe3 to perform Protein Expression Prokaryotic cells - E. coli mycobacterial Esx complex

Get tips on using pMAPLe4 to perform Protein Expression Prokaryotic cells - E. coli mycobacterial Esx complex

Get tips on using pCcrtB to perform Protein Expression Prokaryotic cells - E. coli Kocuria gwangalliensis CrtB

Get tips on using pKUC4 to perform Protein Expression Prokaryotic cells - B. licheniformis PphyL′-′xynA fusion

Get tips on using pKUC3 to perform Protein Expression Prokaryotic cells - B. licheniformis PphyL′-′amyE fusion