sirna-rnai-mirna-transfection-human-primary-splenocytes-polymer-lipid

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Get tips on using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® to perform RNA sequencing Mouse - ESCs (Embryonic Stem Cells)

Products New England BioLabs NEBNext® Multiplex Small RNA Library Prep Set for Illumina®

Get tips on using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® to perform RNA sequencing Mouse - BV-2

Products New England BioLabs NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina®

Get tips on using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® to perform RNA sequencing Mouse - ESCs (Embryonic Stem Cells)

Products New England BioLabs NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina®

Get tips on using "Illumina ™ TotalPrep ™ RNA Amplification Kit + Bio-16-UTP (10 mM) to perform Microarray RNA amplification & Labeling - Mouse cochlaea Biotin

Products Thermo Fisher Scientific "Illumina ™ TotalPrep ™ RNA Amplification Kit + Bio-16-UTP (10 mM)

Get tips on using Enzo BioArray™ Single-Round RNA Amplification and Biotin Labeling System to perform Microarray RNA amplification & Labeling - Rhesus monkey brain tissue Biotin

Products Enzo Life Sciences Enzo BioArray™ Single-Round RNA Amplification and Biotin Labeling System

Get tips on using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® to perform RNA sequencing Mouse - Bone marrow-derived macrophages (BMDMs)

Products New England BioLabs NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina®

Get tips on using Enzo BioArray™ Single-Round RNA Amplification and Biotin Labeling System to perform Microarray Rhesus monkey - Brain tissue Target preparation (RNA amplification + labeling)

Products Enzo Life Sciences Enzo BioArray™ Single-Round RNA Amplification and Biotin Labeling System

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

Discussions Some help with RNA isolation using Trizol

Get tips on using SV Total RNA Isolation System to perform

Products Promega SV Total RNA Isolation System

Get tips on using GeneJET RNA Purification Kit to perform AAA for reviews

Products Thermo Fisher Scientific GeneJET RNA Purification Kit

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